Human Perforin (Active; Human, Natural)
Origin: Cytotoxic granules of YT cells
Molecular Weight: ~70 kDa
Purification: Purified from cytotoxic granules by solubilization in 1M NaCl (Na Acetate buffer) followed by hydrophobic interaction chromatography.
1. Perforin - Liquid in 0.15 M NaCl.
2. Dilution Buffer – 1.0 ml/vial X 23 vials
Specific Activity: 50-150 permeabilizing units (PU)/µg. Cells (1x106/ml) are incubated for 15 min at room temperature with various concentrations of perforin (PFN) and then stained with Propidium iodide (PI). Permeabilized (PI-positive) and non-permeabilized cells are quantified by FACS (or UV light microscopy). One PU is defined as the amount of PFN (ng) that produces 50% PI+ cells in the sample
NOTE: The capacity of PFN to permeabilize nucleated cells varies substantially. The investigator must identify the concentration that provides the desired level of permeabilization for the cell line in question.
Storage and Stability: The stock PFN has to be stored at -80°C. It may be diluted to desired concentration (up to a 10-fold level) with the provided dilution buffer. The diluted PFN may then be sub-aliquoted and frozen (-80°C). The frozen dilute PFN tolerates two freeze-thaw cycles and is stable for up to 3 months. After thaw the diluted PFN is stable at 4°C for approximately 12 hrs.
Handling: For maximum product recovery after thawing, centrifuge the vial before opening the cap.
Limitations: For in vitro research use only. Not for in vitro diagnostic use or use in humans.
Warranty: No warranties, expressed or implied, are made regarding the use of this product.
Country of Origin: USA